Astrid Henning-Jensen worked on a variety of projects during her career. Born in Copenhagen, she began her career as a stage actress until she married Danish filmmaker Bjarne Henning-Jensen in 1938. Astrid began working as his assistant before collaborating with her husband on his films. Their first co-directing and writing credit is the documentary Cykledrengene i Tørvegraven (1941), followed by their short SOS Kindtand (1943). Together with her husband, Astrid made her feature film directorial debut with Those Damned Kids (1947). Throughout the 1940s, the duo made shorts and features together before Astrid made a splash directing independent films solo with the dramatic adaptation Paw (aka Boy of Two Worlds) in 1959. The film was nominated for Best Foreign Language Film at the Academy Awards in 1959.
Henning-Jensen continued to work in film in the 1980s and the 1990s, directing motion pictures like Oejeblikket (1980) and the drama Barndommens gade (1986) with Sofie Grabol. In addition to her directing, Henning-Jensen also appeared on-screen in the Lars Von Trier film The Element of Crime (1987). Henning-Jensen directed her final film in 1996, Bella, min Bella. She passed away in January 2002 at the age of 88.